ABSTRACT Considering the concept of quality of life in cities to be the same, urban planners do not have a precise understanding of the factors affecting the quality of life. Therefore, according to the size of the city, different indicators should be considered to measure the quality of life. Thus, according to the main goal of the research, which is to recognize, identify and compile the factors affecting the improvement of the quality of life in middle and small cities on an intra-provincial scale, Qazvin and Zia Abad cities in Qazvin province were selected as study samples. In order to achieve the goal of the research, exploratory factor analysis and regression analysis were exerted. The findings show that the homogenization of the indicators affecting the quality of life in all cities is incorrect. Some concepts, such as the size of the city, are effective in compiling and selecting the indicators affecting the quality of life. So, the indicators affecting the quality of life in each city can be different according to some concepts, such as the city's characteristics or the city's size. Examining study samples also shows that in small cities, social indicators are more important due to strong social solidarity among people and the high importance of social and cultural concepts. Moreover, in middle cities, due to the importance of finding economic concepts and urban services in people's lives and people's less communication with each other and the weakening of solidarity and social connections, indicators of urban services and facilities and economic factors have the most importance in explaining the quality of life; this shows that the Quality of life indicators are different in all cities Extended Abstract Introduction Quality of life is a multifaceted and dynamic concept that can be different from city to city and region to region and can be defined by various indicators. Not paying attention to this issue and considering the concept of quality of life equally in all cities has caused the level of quality of life to decrease in many cities, especially middle and small cities, in recent years. On the other hand, considering the concept of quality of life in cities to be the same, urban planners do not have a precise understanding of the factors affecting the quality of life. Following this, the main goal of this research is to identify, identify and compile effective factors for improving the quality of life in middle and small cities on an intra-provincial scale. In order to achieve this goal, the cities of Qazvin and Zia Abad in Qazvin province were selected as study samples, so that according to the size of the city, in order to achieve the goal of the research, the factors affecting the quality of life are analyzed on an intra-provincial scale.   Methodology The research method is applied research based on quantitative methods. In line with data analysis, after extracting 60 indicators of quality of life, first, the indicators were separated into separate and structured factors using the exploratory factor analysis method in SPSS software. And then, step by step regression method was used to measure the effective factors in improving the quality of life in cities.   Results and discussion The research findings show that based on exploratory factor analysis, 16 factors were extracted in Zia Abad city and 15 factors in Qazvin city as effective factors on the quality of life in small and middle cities. In order to present and explain the factors affecting the improvement of the quality of life in small and middle cities, the factors extracted from the factor analysis were analyzed by multivariate regression method and step-by-step method. Furthermore, finally, (13) factors were introduced in Zia   Abad and (13) factors in Qazvin as the main factors influencing the quality of life. The analysis of the findings indicates that in Zia Abad, factors such as life expectancy and social relations, security, the state of urban services and access to urban facilities, living expenses, the state of urban furniture, urban traffic, the state of offices and housing have more effects in explaining and improving the quality of life. In Qazvin, factors such as the state of urban services, the state of green and public spaces in the city, the state of security and economic opportunities in the city, the density and hope of urban, economic, and cultural life, the state of roads and leisure in the city are effective in explaining the quality of life. Following this, according to the above findings, quality of life is a multidimensional and dynamic concept that directly relates to the type of cities and the specific characteristics of cities. Therefore, the quality of life and its indicators can differ from city to city; because every city has its characteristics and characteristics with citizens with different cultures and thinking, which causes the factors affecting the quality of life to be different. Therefore, considering the quality of life and its indicators to be the same in all cities is wrong, which can lead to the decline of the quality of life and the failure of quality of life improvement programs.   Conclusion Finally, the research results indicate that quality of life is a simple concept covering various urban life aspects. This concept can be defined as a determining factor in the excellence and progress of cities and residents' satisfaction with their urban life. It is multifaceted and dependent on place and time. As a result, the perception of people in different cities and communities about the concept of quality of life and the factors affecting it can be different. Following this, factors and indicators should be selected according to the characteristics and conditions of that city in urban planning to improve the quality of life for each city. Thus, this research, using the classification of cities based on the size of the city, has presented factors specifically for middle and small cities, which in small cities due to strong social solidarity among people and the great importance of social and cultural concepts, social indicators are more important. Moreover, in middle cities, due to the importance of finding economic concepts and urban services in people's lives and people's less communication with each other and the weakening of solidarity and social connections, indicators of urban services and facilities and economic factors have the most importance in explaining the quality of life; this shows that the quality of life indicators are different in all cities.   Funding There is no funding support.   Authors’ Contribution All of the authors approved the content of the manuscript and agreed on all aspects of the work.   Conflict of Interest Authors declared no conflict of interest.   Acknowledgments We are grateful to all the scientific.

Purification is one of the concepts that has many usage in the literature of education system. Often we use this concept with the known sentence "Purification Precedes Instruction". In this paper, this known sentence is challenged and with the investigation of Koran verses and related interpretations and also from the view of the philosophical discussion of transposition, this precedence is falsified. At the end of paper, we introduce another definition of the concept of purification from two points of views which is in line with the integrative thought on education and instruction.

What is PCR purification.PCR Purification Protocol is designed to purify double stranded DNA fragments from PCR and other enzymatic reactions. Fragments from 100bp to 10kb are purified from primers, nucleotides, polymerases and salts using QIAquick spin columns in a microcentrifuge. Even if distinct bands of the expected size are observed, primer dimers should be removed by PCR purification method. In the absence of the purification, the proofreading activity of DNA polymerase will degrade the PCR fragments or remove the 3' terminal deoxyadenosine from the vector during ligation reaction.

OBJECTIVES: Anti-lymphocyte globulin (ALG) is a polyc1onal anti human lymphocyte surface markers which is produced in rabbits. ALG is one of the potent immunosuppressive agents which is currently used for preventing organ allograft rejection, treatment of aplastic anemia and some autoimmune disorders. ALG causes elimination of human lymphocytes from the peripheral blood circulation, regulation of cytotoxic activities and apoptosis. The goal of this project was urification of ALG by a simple, rapid and inexpensive method, ion exchange chromatography. METHODS: ALG riched serum of immunized rabbit with human lymphocytes was diluted with phosphate-buffered saline (PBS, pH 7.4) in a ratio of 1:1 and precipitated with ammonium sulfate in the final concentration of 50%. The precipitant was rewashed with 50% ammonium sulfate, dialyzed against tris-phosphate pH=8.1 and applied to ion-exchange chromatography on DEAE-Sepharose 6B.ALG riched fraction was eluted with starting buffer; Tris-Phosphate (PH=8.1) and final buffer; Tris-Phosphate containing 50 mM NaCl (PH=8.1). RESULTS: The purity of ALG with high percent purity was confirmed by SDS-PAGE, and efficiency of it by histocompatibility assay. CONCLUSION: This preparation is comparable in purity to those of commercially available standard ALG in the benefit of self sufficiency. The 1/80 dilution of purified ALG can lyse human lymphocytes properly in histocompatibility testing which verifies its high efficiency.